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1996-02-27
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Document 0400
DOCN M9630400
TI Capillary and slab gel electrophoresis profiling of oligosaccharides.
DT 9603
AU Guttman A; Starr C; Beckman Instruments, Inc, Fullerton, CA 92634, USA.
SO Electrophoresis. 1995 Jun;16(6):993-7. Unique Identifier : AIDSLINE
MED/96118594
AB Capillary electrophoresis with laser-induced fluorescent detection, a
one-dimensional version of the well-established planar analytical method
of polyacrylamide gel electrophoresis, has been proven to be a powerful
new microanalytical method for profiling complex carbohydrates. In this
paper a comparison is presented between the planar high concentration
polyacrylamide gel electrophoresis method and capillary electrophoresis
of different carbohydrates with respect to performance and efficiency.
N-Linked oligosaccharides were released from several glycoproteins,
including fetuin, human immunodeficiency virus (HIV) envelope
recombinant glycoprotein (GP-120), alpha 1-acid glycoprotein and
ribonuclease B, using recombinant peptide-N-glycosidase F (PNGase F).
Both separation methods involve labeling of the released carbohydrates
at the reducing end with the fluorescent dye, disodium
8-amino-1,3,6-naphthalene trisulfonate (ANTS). Fluorophore labeling was
followed by separation of the labeled oligosaccharides either by high
concentration polyacrylamide gel electrophoresis or capillary
electrophoresis.
DE Animal Cattle Comparative Study Electrophoresis, Capillary/*METHODS
Electrophoresis, Polyacrylamide Gel/*METHODS Glycoproteins/CHEMISTRY
Human Oligosaccharides/*ANALYSIS JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).